Journal: Oncology Letters

Article Title: Significance of interstitial tumor-associated macrophages in the progression of lung adenocarcinoma

doi: 10.3892/ol.2016.5270

Figure Lengend Snippet: Typical expression images of immunochemistry staining in tissue microarrays. (A and B) Low expression of CD68 in MIA tissues. Original magnification, (A) ×100 and (B) ×200. (C and D) High expression of CD68 in LPA tissues. Original magnification, (C) ×100 and (D) ×200. (E) Negative expression of IL-6 in MIA tissues. Original magnification, ×200. (F) Positive expression of IL-6 in LPA tissues. Original magnification, ×200. (G) Negative expression of CSF-1 in MIA tissues. Original magnification, ×200. (H) Positive expression of CSF-1 in LPA tissues. Original magnification, ×200. (I) High E-cadherin expression in MIA tissues. Original magnification, ×100. (J) Low E-cadherin expression in LPA tissues. Original magnification, ×100. (K) High Snail expression in LPA tissues. Original magnification, ×100. (L) High MMP-2 expression in LPA tissues. Original magnification, ×100. CD, cluster of differentiation; MIA, minimally invasive adenocarcinoma; LPA, lepidic predominant adenocarcinoma; CSF, colony-stimulating factor; IL, interleukin; MMP, metalloproteinase.

Article Snippet: Immunohistochemistry was performed with mouse anti-human CD68 monoclonal antibody at 1:5 dilution (clone KP1; Abcam, Cambridge, UK), rabbit anti-human colony-stimulating factor (CSF)-1 polyclonal antibody at 1:100 dilution (BA0750; Wuhan Boster Biological Technology, Ltd., Wuhan, China), anti-interleukin (IL)-6 at 1:100 dilution (BS0781R; BIOSS, Beijing, China), anti-matrix metalloproteinase (MMP)-2 at 1:400 dilution (ab37150; Abcam), anti-E-cadherin at 1:200 dilution (sc-7,870; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and anti-Snail at 1:25 dilution (AP2054a; Abgent, Inc., San Diego, CA, USA).

Techniques: Expressing, Staining

Journal: Oncology Letters

Article Title: Significance of interstitial tumor-associated macrophages in the progression of lung adenocarcinoma

doi: 10.3892/ol.2016.5270

Figure Lengend Snippet: (A) Kaplan-Meier analysis of overall survival for the combination of CD68, CSF-1 and IL-6. (B) Kaplan-Meier analysis of disease-free survival for the combination of CD68, CSF-1 and IL-6. CSF-1, colony-stimulating factor-1; CD, cluster of differentiation; IL, interleukin,

Article Snippet: Immunohistochemistry was performed with mouse anti-human CD68 monoclonal antibody at 1:5 dilution (clone KP1; Abcam, Cambridge, UK), rabbit anti-human colony-stimulating factor (CSF)-1 polyclonal antibody at 1:100 dilution (BA0750; Wuhan Boster Biological Technology, Ltd., Wuhan, China), anti-interleukin (IL)-6 at 1:100 dilution (BS0781R; BIOSS, Beijing, China), anti-matrix metalloproteinase (MMP)-2 at 1:400 dilution (ab37150; Abcam), anti-E-cadherin at 1:200 dilution (sc-7,870; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and anti-Snail at 1:25 dilution (AP2054a; Abgent, Inc., San Diego, CA, USA).

Techniques:

Journal: Oncology Letters

Article Title: Significance of interstitial tumor-associated macrophages in the progression of lung adenocarcinoma

doi: 10.3892/ol.2016.5270

Figure Lengend Snippet: (A) OS in patients with AIS/MIA in the CD68+CSF-1+IL-6+ group compared with other groups, including the CD68-, CD68+CSF-1+IL-6-, CD68+CSF-1-IL-6+ and CD68+CSF-1-IL-6- groups. (B) DFS in patients with AIS/MIA in the CD68+ CSF-1+ IL-6+ group compared with other groups (C) Comparison of OS in AIS/MIA patients with CD68+CSF-1+IL-6+ and in LPA patients with CD68+CSF-1+IL-6+. (D) Comparison of DFS in AIS/MIA patients with CD68+CSF-1+IL-6+ and in LPA patients with CD68+CSF-1+IL-6+. *Others include the CD68+CSF-1+IL-6- group, the CD68+CSF-1-IL-6+ group, the CD68+CSF-1-IL-6- group and the CD68- group. AIS, adenocarcinoma in situ ; MIA, minimally invasive adenocarcinoma; CD, cluster of differentiation; CSF, colony-stimulating factor; IL, interleukin; OS, overall survival; DFS, disease-free survival.

Article Snippet: Immunohistochemistry was performed with mouse anti-human CD68 monoclonal antibody at 1:5 dilution (clone KP1; Abcam, Cambridge, UK), rabbit anti-human colony-stimulating factor (CSF)-1 polyclonal antibody at 1:100 dilution (BA0750; Wuhan Boster Biological Technology, Ltd., Wuhan, China), anti-interleukin (IL)-6 at 1:100 dilution (BS0781R; BIOSS, Beijing, China), anti-matrix metalloproteinase (MMP)-2 at 1:400 dilution (ab37150; Abcam), anti-E-cadherin at 1:200 dilution (sc-7,870; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and anti-Snail at 1:25 dilution (AP2054a; Abgent, Inc., San Diego, CA, USA).

Techniques: In Situ

Journal: bioRxiv

Article Title: A Consequence of Immature Breathing induces Persistent Changes in Hippocampal Synaptic Plasticity and Behavior: A Role of Pro-Oxidant State and NMDA Receptor Imbalance

doi: 10.1101/2023.03.21.533692

Figure Lengend Snippet: A . ( left ) Representative traces of the evoked fEPSP from control (black), control+AP5 [50µM] (green), nIH (red) and nIH +AP5 [50µM] (blue) in baseline conditions prior to TBS (1) and following TBS (2). ( middle ) Mean fEPSP slope plotted as a function of time relative to the slope before TBS in control, control+AP5, nIH and nIH+AP5. ( right ) fEPSP slope represented as percent change from baseline at 60 min after TBS in control slices vs control+AP5 [50 µM]. B . ( top ) Representative image for GluN1. (bottom) The graph shows GluN1 did not change protein expression after nIH exposure compared with control. (two tailed t-test, t=0.63; df=4.1; P=0.55). C . (top) Western blot picture for GluN2A. (bottom) Comparison for both conditions show GluN2A decreased the protein content levels after nIH. (two tailed t-test, t=4.017; df=6.43; P=0.006). D . ( top ) Representative immunoblot image for GluN2B. (bottom) Comparison of both conditions show increased GluN2B levels after nIH (two tailed t-test, t=3.43; df=5.78; P=0.014). E . GluN2B/GluN2A comparison ratio. F . ( left ) Representative traces of the evoked fEPSP control +TCN [5 µM] (orange), control + ifenprodil [5 µM] (cyan) and control + both drugs (olive) in baseline conditions prior to TBS (1) and following TBS (2). ( middle ) Mean fEPSP slope plotted as a function of time relative to the slope before TBS and ( right ) Comparison of fEPSP slope represented as percent change from baseline at 60 min after TBS (one way ANOVA, F (3,20) =43.52; P<0.001). Black dashed line represents the mean slope of the fEPSP 60 min following TBS in control slices from . G . ( left ) Representative traces of the evoked fEPSP from nIH+TCN [5 µM] (dark yellow) and nIH +ifenprodil [5 µM] (light blue) in baseline conditions prior to TBS (1) and following TBS (2). ( middle ) Mean fEPSP slope plotted as a function of time and relative to slope before TBS ( right ) fEPSP slope represented as percent change from baseline at 60 min after TBS (two tailed t-test, t=12.46; df=4.59; P=0.001). Red dashed line represents the mean slope of the fEPSP 60 min following TBS in nIH slices from . Scale bars for A, F and G: 10 msec x 0.2 mV. The box-plot parameters indicate mean ± S.E. The analysis was performed for A to E using unpaired two-tailed t-test with Welch’s correction and for F and G the analysis was performed using one-way ANOVA followed by Bonferroni post hoc. **P<0.01, ***P<0.001 and ****P<0.0001).

Article Snippet: Membranes were incubated under constant shaking with primary antibodies: anti rabbit GluN1 (1:2000; Abcam Cat# ab109182, RRID:AB_10862307) anti-rabbit GluN2A (1:2000; Cell Signaling Technology Cat# 4205, RRID:AB_2112295), anti-rabbit GluN2B (1:2000; Cell Signaling Technology Cat# 14544, RRID:AB_2798506), anti-rabbit NOX2 (1:2000; Abcam Cat# ab129068, RRID:AB_11144496) anti-rabbit NOX4 (1:500; Novus Cat# NB110-58851B, RRID:AB_1217375 and anti-mouse GAPDH (1: 10.000; Abcam Cat# ab8245, RRID:AB_2107448).

Techniques: Control, Expressing, Two Tailed Test, Western Blot, Comparison

Journal: bioRxiv

Article Title: A Consequence of Immature Breathing induces Persistent Changes in Hippocampal Synaptic Plasticity and Behavior: A Role of Pro-Oxidant State and NMDA Receptor Imbalance

doi: 10.1101/2023.03.21.533692

Figure Lengend Snippet: A . Malondialdehyde (MDA) content was measured in hippocampal homogenates from control, nIH Saline and 10-Mn. (one way ANOVA, F (2,12) =11.53; P=0.0016). B . (top) Representative blot of nuclear HIF1a performed from control, nIH Saline and IH 10-Mn mice. (bottom) Quantification of HIF1a expression (one way ANOVA, F (2,12) =5.76; P=0.017). C . (top) Immunoblot of NOX2. (bottom) Significant differences was found in hippocampal homogenate from nIH Saline compared to control and nIH Mn (one way ANOVA, F (2,15) =9.26; P=0.0024). D . (top) Representative image of NOX4. ( bottom ) Comparison of NOX4 expression between control, nIH Saline and nIH Mn . (one way ANOVA, F (2,9) =11.46; P=0.0034). E . ( left ) Representative blot of GluN2A from control, nIH Saline and nIH Mn mice. ( right ). Quantification of GluN2A expression. (one way ANOVA, F (2,15) =6.63; P=0.0086). F . ( left ) Immunoblot of GluN2B. ( right ) Significant differences were found in hippocampal homogenate from nIH Saline vs control and nIH Mn . (one way ANOVA, F (2,12) =6.88; P=0.01). G . (top) Representative traces of evoked fEPSP from nIH Mn (purple) in baseline conditions prior to TBS (1) and following TBS (2). ( left bottom ) Mean fEPSP slope plotted as a function of time relative to the slope before TBS in nIH Mn . ( right ) fEPSP slope represented as percent change from baseline at 60 min after TBS in nIH vs nIH Mn slices. (two tailed t-test, t=3.41; df=10.61; P=0.0061). Dashed lines represent the mean slope of the fEPSP 60 min following TBS in control (black dashed line) and nIH (red dashed line) slices from . H . (top) Representative traces of the evoked fEPSP from nIH Mn in presence the ifenprodil [5 µM] (light purple) in baseline conditions prior to TBS (1) and following TBS (2). ( left bottom ) Mean fEPSP slope plotted as a function of time relative to the slope before TBS in nIH Mn in presence the ifenprodil. ( right ) fEPSP slope represented as percent change from baseline at 60 min after TBS in IH+ifenprodil vs nIH Mn in presence of ifenprodil. (two tailed t-test, t=3.99; df=6.99; P=0.019). Blue dashed line represents the mean slope of the fEPSP 60 min following TBS in ifenprodil treated nIH slices from . For G and H, Scale bars 10 msec x 0.2 mV. The analysis was performed for A to F using one-way ANOVA followed by Bonferroni post hoc and for G and F, the analysis was performed using unpaired two-tailed t-test with Welch’s correction. *P<0.05, **P<0.01, and ***P<0.001.

Article Snippet: Membranes were incubated under constant shaking with primary antibodies: anti rabbit GluN1 (1:2000; Abcam Cat# ab109182, RRID:AB_10862307) anti-rabbit GluN2A (1:2000; Cell Signaling Technology Cat# 4205, RRID:AB_2112295), anti-rabbit GluN2B (1:2000; Cell Signaling Technology Cat# 14544, RRID:AB_2798506), anti-rabbit NOX2 (1:2000; Abcam Cat# ab129068, RRID:AB_11144496) anti-rabbit NOX4 (1:500; Novus Cat# NB110-58851B, RRID:AB_1217375 and anti-mouse GAPDH (1: 10.000; Abcam Cat# ab8245, RRID:AB_2107448).

Techniques: Control, Saline, Expressing, Western Blot, Comparison, Two Tailed Test

Journal: bioRxiv

Article Title: A Consequence of Immature Breathing induces Persistent Changes in Hippocampal Synaptic Plasticity and Behavior: A Role of Pro-Oxidant State and NMDA Receptor Imbalance

doi: 10.1101/2023.03.21.533692

Figure Lengend Snippet: A . (top) Representative traces of the evoked fEPSP from adult control (black) and adult mice were exposed to neonatal IH (red) in baseline conditions prior to TBS (1) and following TBS (2). ( left bottom ) Mean fEPSP slope plotted as a function of time and relative to baseline prior to TBS. ( right bottom ) fEPSP slope represented as percent change from baseline at 60 min following TBS in adult control vs Adult nIH (two tailed t-test, t=5.70; df=9.04; P=0.003). B . (top) Representative trace of the evoked from control+ TCN-213 [5 µM] and control + ifenprodil [5 µM] in baseline conditions prior to TBS (1) and following TBS (2). ( left bottom ) Mean fEPSP slope plotted as a function of time and relative to baseline prior to TBS. ( right bottom ) fEPSP comparison at 60 min following TBS in control +TCN and control+ ifenprodil (two tailed t-test, t=7.43; df=4.65; P=0.0009). Black dashed line represents the mean slope of the fEPSP 60 min following TBS in control slices from . C . (top) Representative trace of the evoked response from Adult nIH + TCN-213 [5 µM] and Adult nIH + ifenprodil [5 µM] in baseline conditions prior to TBS (1) and following TBS (2). ( left bottom ) Mean fEPSP slope plotted as a function of time relative to baseline prior to TBS. ( right bottom ) fEPSP slope represented as percent change from baseline at 60 min following TBS (two tailed t-test, t=4.72; df=4.06; P=0.0064). Red dashed line represents the mean slope of the fEPSP after 60 min following TBS in control slices from . D . (top) Representative traces of the evoked fEPSP from adult mice was exposure to neonatal IH+10 days of MnTMPyP (Adult nIH-Mn ) and adult mice receive MnTMPyP after exposure to neonatal IH (Adult REC-Mn ) in baseline conditions before TBS (1) and after TBS (2). ( left bottom ) Mean fEPSP slope plotted as a function of time and relative to slope before TBS in Adult nIH-Mn vs Adult REC-Mn ( right bottom ) fEPSP slope represented as percent change from baseline at 60 min after TBS Adult nIH-Mn vs Adult REC-Mn (two tailed t-test, t=3.79; df=8.16; P=0.0051). E. Representative image of GluN1. ( bottom ) Quantification shows GluN1 protein expression is not changed in Adult nIH , Adult nIH-Mn or 10+Mn exposures compared to control (one way ANOVA, F (3,16)=1.14 ; P=0.93; N=5). F . (top) Representative blot of GluN2A performed from adult mice unexposed, Adult nIH , Adult nIH-Mn or Adult REC-Mn . ( bottom ). Quantification of GluN2A expression from adult control, Adult nIH , Adult nIH-Mn or 10+Mn. (one way ANOVA, F (3,12)=8.31 ; P=0.0029, N=4). G . (top) Immunoblot of GluN2B. ( bottom ) Significant differences were found in hippocampal homogenates from adult control, Adult nIH , Adult nIH-Mn or Adult REC-Mn (one way ANOVA, F (3,12)=4.91 ; P=0.018, N=4). The box plot parameters indicate mean ± S.E. The analysis was performed for A-D using unpaired two-tailed t-test with Welch’s correction. The analysis was performed for E-G using one-way ANOVA followed by Bonferroni post hoc. *P=0.05, **P=0.01, ***P=0.001 and N.S= no significant. Scale bars for A,B, F and G= 10 msec x 0.2 mV

Article Snippet: Membranes were incubated under constant shaking with primary antibodies: anti rabbit GluN1 (1:2000; Abcam Cat# ab109182, RRID:AB_10862307) anti-rabbit GluN2A (1:2000; Cell Signaling Technology Cat# 4205, RRID:AB_2112295), anti-rabbit GluN2B (1:2000; Cell Signaling Technology Cat# 14544, RRID:AB_2798506), anti-rabbit NOX2 (1:2000; Abcam Cat# ab129068, RRID:AB_11144496) anti-rabbit NOX4 (1:500; Novus Cat# NB110-58851B, RRID:AB_1217375 and anti-mouse GAPDH (1: 10.000; Abcam Cat# ab8245, RRID:AB_2107448).

Techniques: Control, Two Tailed Test, Comparison, Expressing, Western Blot